Extrusion of cyclic AMP from pigeon erythrocytes.

Compartmentalization of cyclic AMP-dependent protein kinases in human erythrocytes.

The human erythrocyte contains two types of cyclic AMP-dependent protein kinase. The membrane-associated protein kinase holoenzyme can be released intact by hypotonic treatment at alkaline pH. Chromatography on DEAE-cellulose and molecular weight determinations demonstrate that this enzyme is a type I cyclic AMP-dependent protein kinase, while the predominant cyclic AMP-dependent protein kinase...

آدنوزین منوفسفات حلقوی ( Cyclic AMP )

Turnover of Phosphatidic Acid and Sodium Extrusion from Mammalian Erythrocytes

Phosphatidic acid (PA) from swine and beef RBCs was isolated by chromatography on silicic acid columns. It comprised about 1 per cent of the total lipid phosphate in RBCs, but was eluted nearly pure from columns. An uncharacterized inositide accounted for 5 to 10 per cent of the phosphate in the PA-containing fraction. When cells were incubated with HP(32)O(4) (=), the fraction containing PA be...

Effect of norepinephrine and hypertonicity on K influx and cyclic AMP in duck erythrocytes.

Cyclic adenosine 3',5'-monophosphate (cAMP) accumulation and cation transport were measured in duck erythrocytes after stimulation by norepinephrine (NE) or shrinkage induced by exposure to hypertonic media (S). Previously both NE and S were shown to initiate a similar transport process in this cell. NE elicited a rapid rise in cellular cAMP and 42K influx. Both effects were eliminated by propr...

Numerical investigation of strain distribution during cyclic expansion extrusion (CEE)

Strain distribution of Al 1100 was numerically investigated during cyclic expansion extrusion (CEE) process using finite element method (FEM). Die angle, corner fillet radius and die chamber diameter were considered as die parameters and friction factor and number of passes as process parameters. The effects of these parameters were investigated on the effective strain and strain homogeneity in...